The findings, outlined in the June 24 early edition of the Proceedings of the National Academy of Sciences (PNAS), strongly support the idea that manganese oxidation — which, despite the name, is a chemical reaction that does not have to involve oxygen — provided an evolutionary stepping-stone for the development of water-oxidizing photosynthesis in cyanobacteria.

“Water-oxidizing or water-splitting photosynthesis was invented by cyanobacteria approximately 2.4 billion years ago and then borrowed by other groups of organisms thereafter,” explains Woodward Fischer, assistant professor of geobiology at Caltech and a coauthor of the study. “Algae borrowed this photosynthetic system from cyanobacteria, and plants are just a group of algae that took photosynthesis on land, so we think with this finding we’re looking at the inception of the molecular machinery that would give rise to oxygen.”

Photosynthesis is the process by which energy from the sun is used by plants and other organisms to split water and carbon dioxide molecules to make carbohydrates and oxygen. Manganese is required for water splitting to work, so when scientists began to wonder what evolutionary steps may have led up to an oxygenated atmosphere on Earth, they started to look for evidence of manganese-oxidizing photosynthesis prior to cyanobacteria. Since oxidation simply involves the transfer of electrons to increase the charge on an atom — and this can be accomplished using light or O₂ — it could have occurred before the rise of oxygen on this planet.

“Manganese plays an essential role in modern biological water splitting as a necessary catalyst in the process, so manganese-oxidizing photosynthesis makes sense as a potential transitional photosystem,” says Jena Johnson, a graduate student in Fischer’s laboratory at Caltech and lead author of the study.

To test the hypothesis that manganese-based photosynthesis occurred prior to the evolution of oxygenic cyanobacteria, the researchers examined drill cores (newly obtained by the Agouron Institute) from 2.415 billion-year-old South African marine sedimentary rocks with large deposits of manganese.

Manganese is soluble in seawater. Indeed, if there are no strong oxidants around to accept electrons from the manganese, it will remain aqueous, Fischer explains, but the second it is oxidized, or loses electrons, manganese precipitates, forming a solid that can become concentrated within seafloor sediments.

“Just the observation of these large enrichments — 16 percent manganese in some samples — provided a strong implication that the manganese had been oxidized, but this required confirmation,” he says.

To prove that the manganese was originally part of the South African rock and not deposited there later by hydrothermal fluids or some other phenomena, Johnson and colleagues developed and employed techniques that allowed the team to assess the abundance and oxidation state of manganese-bearing minerals at a very tiny scale of 2 microns.

“And it’s warranted — these rocks are complicated at a micron scale!” Fischer says. “And yet, the rocks occupy hundreds of meters of stratigraphy across hundreds of square kilometers of ocean basin, so you need to be able to work between many scales — very detailed ones, but also across the whole deposit to understand the ancient environmental processes at work.”

Using these multiscale approaches, Johnson and colleagues demonstrated that the manganese was original to the rocks and first deposited in sediments as manganese oxides, and that manganese oxidation occurred over a broad swath of the ancient marine basin during the entire timescale captured by the drill cores.

“It’s really amazing to be able to use X-ray techniques to look back into the rock record and use the chemical observations on the microscale to shed light on some of the fundamental processes and mechanisms that occurred billions of years ago,” says Samuel Webb, coauthor on the paper and beam line scientist at the SLAC National Accelerator Laboratory at Stanford University, where many of the study’s experiments took place. “Questions regarding the evolution of the photosynthetic pathway and the subsequent rise of oxygen in the atmosphere are critical for understanding not only the history of our own planet, but also the basics of how biology has perfected the process of photosynthesis.”

Once the team confirmed that the manganese had been deposited as an oxide phase when the rock was first forming, they checked to see if these manganese oxides were actually formed before water-splitting photosynthesis or if they formed after as a result of reactions with oxygen. They used two different techniques to check whether oxygen was present. It was not — proving that water-splitting photosynthesis had not yet evolved at that point in time. The manganese in the deposits had indeed been oxidized and deposited before the appearance of water-splitting cyanobacteria. This implies, the researchers say, that manganese-oxidizing photosynthesis was a stepping-stone for oxygen-producing, water-splitting photosynthesis.

“I think that there will be a number of additional experiments that people will now attempt to try and reverse engineer a manganese photosynthetic photosystem or cell,” Fischer says. “Once you know that this happened, it all of a sudden gives you reason to take more seriously an experimental program aimed at asking, ‘Can we make a photosystem that’s able to oxidize manganese but doesn’t then go on to split water? How does it behave, and what is its chemistry?’ Even though we know what modern water splitting is and what it looks like, we still don’t know exactly how it works. There is a still a major discovery to be made to find out exactly how the catalysis works, and now knowing where this machinery comes from may open new perspectives into its function — an understanding that could help target technologies for energy production from artificial photosynthesis. ”

Next up in Fischer’s lab, Johnson plans to work with others to try and mutate a cyanobacteria to “go backwards” and perform manganese-oxidizing photosynthesis. The team also plans to investigate a set of rocks from western Australia that are similar in age to the samples used in the current study and may also contain beds of manganese. If their current study results are truly an indication of manganese-oxidizing photosynthesis, they say, there should be evidence of the same processes in other parts of the world.

“Oxygen is the backdrop on which this story is playing out on, but really, this is a tale of the evolution of this very intense metabolism that happened once — an evolutionary singularity that transformed the planet,” Fischer says. “We’ve provided insight into how the evolution of one of these remarkable molecular machines led up to the oxidation of our planet’s atmosphere, and now we’re going to follow up on all angles of our findings.”

Funding for the research outlined in the PNAS paper, titled “Manganese-oxidizing photosynthesis before the rise of cyanobacteria,” was provided by the Agouron Institute, NASA’s Exobiology Branch, the David and Lucile Packard Foundation, and the National Science Foundation Graduate Research Fellowship program. Joseph Kirschvink, Nico and Marilyn Van Wingen Professor of Geobiology at Caltech, also contributed to the study along with Katherine Thomas and Shuhei Ono from the Massachusetts Institute of Technology.

The study, in the Nature journal Scientific Reports, demonstrates that mid-latitude high pressure zones (30^oS-45^oS) are being pushed further into the Southern Ocean by rising global temperatures associated with greenhouse warming. This is despite more frequent occurrences of strong El Niños in recent decades, which should have drawn the high pressure zones in the opposite direction toward the equator.

“What we are seeing,” says study lead author, Mr Guojian Wang “is a ‘tug of war’ between stronger El Niños driving the winds north and the greenhouse gas-warming effect driving the winds south.”

Mr Wang, said the result confirms the robustness of the Southern Hemisphere circulation changes over the past three to four decades as the global temperature rose, “so much so that it overode the influence from strong El Niños during this period.”

Study co-author, Dr Wenju Cai said the most conspicuous change is a rising sea level pressure in the mid-latitude bands and a decreasing sea level pressure over the Southern high latitudes (55^o-70^oS), a pattern referred to as the Southern Annular Mode. The changing pressures indicate a poleward or southward expansion of the tropical and subtropical atmospheric zones.

In turn, this indicates that over the long-term, there is a relationship between a rising global mean temperature and an upward trend of the Southern Annular Mode.

“The research reinforces our past work that climate change is altering Southern Hemisphere circulation and increases our confidence in this conclusion,” Dr Cai said.

Dr Cai has previously reported on changes in atmospheric circulation that have been shifting and strengthening the Pacific Ocean winds poleward and in turn strengthening the ocean circulation, pushing the East Australian Current further south down the Australian coast.

He said during El Niño, the warmer ocean releases heat to the atmosphere and global average temperatures increase. At the same time, warm ocean surface temperatures along the equator cause the tropical and subtropical atmospheric belts to move toward the equator, generating a ‘negative’ phase of the Southern Annular Mode.

“On year-to-year time scales, higher global temperatures are associated with a negative phase of the Mode but over the past 35 years, when El Niño has been strong and conducive to a negative trend, we are seeing an opposite trend with the circulation systems moving southward impacting on regional climate,” he said.

The project was funded through the Australian Climate Change Science Program.

Conrad has studied patterns of tectonic plates throughout his career, and has long noticed that the plates were, on average, moving northward. “Knowing this,” explained Conrad, “I was curious if I could determine a single location in the Northern Hemisphere toward which all plates are converging, on average.” After locating this point in eastern Asia, Conrad then wondered if other special points on Earth could characterize plate tectonics. “With some mathematical work, I described the plate tectonic ‘quadrupole’, which defines two points of ‘net convergence’ and two points of ‘net divergence’ of tectonic plate motions.”

When the researchers computed the plate tectonic quadruople locations for present-day plate motions, they found that the net divergence locations were consistent with the African and central Pacific locations where scientists think that mantle upwellings are occurring today. “This observation was interesting and important, and it made sense,” said Conrad. “Next, we applied this formula to the time history of plate motions and plotted the points — I was astonished to see that the points have not moved over geologic time!” Because plate motions are merely the surface expression of the underlying dynamics of the Earth’s mantle, Conrad and his colleagues were able to infer that upwelling flow in the mantle must also remain stable over geologic time. “It was as if I was seeing the ‘ghosts’ of ancient mantle flow patterns, recorded in the geologic record of plate motions!”

Earth’s mantle dynamics govern many aspects of geologic change on the Earth’s surface. This recent discovery that mantle upwelling has remained stable and centered on two locations (beneath Africa and the Central Pacific) provides a framework for understanding how mantle dynamics can be linked to surface geology over geologic time. For example, the researchers can now estimate how individual continents have moved relative to these two upwelling locations. This allows them to tie specific events that are observed in the geologic record to the mantle forces that ultimately caused these events.

More broadly, this research opens up a big question for solid earth scientists: What processes cause these two mantle upwelling locations to remain stable within a complex and dynamically evolving system such as the mantle? One notable observation is that the lowermost mantle beneath Africa and the Central Pacific seems to be composed of rock assemblages that are different than the rest of the mantle. Is it possible that these two anomalous regions at the bottom of the mantle are somehow organizing flow patterns for the rest of the mantle? How?

“Answering such questions is important because geologic features such as ocean basins, mountains belts, earthquakes and volcanoes ultimately result from Earth’s interior dynamics,” Conrad described. “Thus, it is important to understand the time-dependent nature of our planet’s interior dynamics in order to better understand the geological forces that affect the planetary surface that is our home.”

The mantle flow framework that can be defined as a result of this study allows geophysicists to predict surface uplift and subsidence patterns as a function of time. These vertical motions of continents and seafloor cause both local and global changes in sea level. In the future, Conrad wants to use this new understanding of mantle flow patterns to predict changes in sea level over geologic time. By comparing these predictions to observations of sea level change, he hopes to develop new constraints on the influence of mantle dynamics on sea level.